Yes, but the process is a little more complicated than a typical rollover. Moving money from a traditional 401(k) to a Roth IRA is usually a two-step process: first, you’ll move your Traditional 401(k) to a Traditional IRA. Then, your IRA provider will move your Traditional IRA into a Roth IRA (this is known as a “Roth Conversion”).
Keep in mind that most Roth conversions are taxable, so it’s a good idea to talk to a tax professional if you decide to go down this path.